高级检索

模拟干旱胁迫对油菜KN DH群体农艺性状和生理指标的影响

Effects of Simulated Drought Stress on Related Traits and Physiological Indicators of KN DH Population in Brassica napus

  • 摘要: 为筛选、鉴定出耐旱性油菜优良种质资源,以甘蓝型油菜KN DH群体为试验材料,利用改良1/2的Hoagland完全营养液,通过在营养液中添加不同浓度的PEG-6000(5%、10%、15%、20%、25%)模拟干旱胁迫,探究模拟干旱胁迫对油菜KN DH群体农艺性状及生理指标的影响,并对干旱胁迫下其相关性状和主成分进行了分析。结果表明:(1)20%PEG-6000浓度模拟干旱胁迫处理为油菜KN DH群体的适宜胁迫浓度。(2)在干旱胁迫处理油菜幼苗的农艺性状中,茎叶鲜重的变异系数最大,生理指标中过氧化氢酶活性的变异系数增幅最大。(3)在20%PEG-6000处理后,油菜最大根长与株高、茎叶鲜重、根系鲜重、过氧化氢酶活性呈显著正相关,与根冠比、丙二醛含量呈显著负相关;茎叶鲜重、过氧化氢酶活性与丙二醛含量呈显著负相关。(4)5个主成分因子(根系鲜重因子、茎叶鲜重因子、过氧化氢酶因子、叶绿素因子和丙二醛因子)累计贡献率达到86.82%,基本反映了调查性状的全部信息。

     

    Abstract: The study aims to reveal the effects ofsimulated drought stress on related agronomic traits and physiological indicators of KN DH population in rapeseed, thereby laying a foundation for the screening and identification of drought-tolerant rapeseed germplasm resources and the breeding of excellent varieties. The 1/2 Hoagland complete nutrient solution with different concentrations(5%, 10%, 15%, 20% and 25%) of polyethylene glycol 6000(PEG-6000) was used to simulate drought stress of KN DH population, the effects, the correlation analysis and principal component analysis were performed in study. The results showed that:(1) The PEG-6000 concentration of 20% in the nutrient solution was suitable for simulated drought stress in the KN DH population parents.(2) The(coefficient of variation) of stem and leaf fresh weight was the highest, the CV of CAT increased the most.(3) After drought stress treatment with 20% PEG-6000, maximum root length was positively correlated with plant height, stem and leaf fresh weight, root fresh weight and CAT, but was negatively correlated with root-shoot ratio and MDA content. The stem and leaf fresh weight and CAT were significantly negatively correlated with MDA content.(4) Eight traits could be integrated into five principal component factors(root fresh weight, stem and leaf fresh weight, CAT, chlorophyll and MDA), with the total cumulative contribution rate of 86.82%, basically reflecting all the information of the investigated traits.