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基于HS-SPME-GC-MS和电子鼻分析不同干燥方式对茶树花茶挥发性成分的影响

Effect of Different Drying Methods on Volatile Components of Flower Tea of Camellia sinensis Based on HS-SPME-GC-MS and Electronic Nose Methods

  • 摘要: 为探究不同干燥方式对茶树花茶挥发性成分的影响,以炒制干燥(SFD)、热风干燥(HAD)、微波干燥(MD)和真空干燥(VD)4种不同干燥方式加工的茶树花茶为原料,采用感官评价、电子鼻与顶空固相微萃取—气相色谱—质谱联用技术(HS-SPME-GC-MS),综合分析了不同干燥方式加工的茶树花茶挥发性成分。结果表明,不同干燥方式对茶树花茶挥发性成分的影响存在显著差异。SFD呈甜香、HAD呈清香、MD呈蜜香、VD呈果香;电子鼻结果初步表明HAD与SFD茶树花茶香气较为相似;HS-SPME-GC-MS技术共检测到113种香气成分,醛类化合物25种、醇类化合物44种、酮类化合物22种、酯类化合物9种、碳氢化合物10种、其他化合物3种,挥发性成分含量整体表现为HAD>MD>VD>SFD。通过PLS-DA及差异显著性分析,筛选出重要的22种不同干燥方式中茶树花茶挥发性成分差异的显著性标记物,并结合气味活度值(OAV>1)筛选出15种关键物质(苏合香醇、己醛、苯乙酮、芳樟醇、庚醛、2-甲基丁醛、1-戊烯-3-酮、(E)-2-辛烯醛、糠醛、芳樟醇氧化物Ι、壬醇、壬醛、芳樟醇氧化物Ⅲ、香叶醇、紫罗兰酮);另外,4种干燥方式加工的茶树花茶都包含且OAV值均大于10的有紫罗兰酮、2-甲基丁醛、(E,E)-2,4-癸二烯醛、1-辛烯-3-醇,这些香气物质是茶树花茶的关键挥发性成分。不同干燥方式加工的茶树花茶挥发性成分在种类上相似,而在含量上差异显著;从挥发性成分含量上看,HAD有利于茶树花茶挥发性成分的产生和累积。

     

    Abstract: In order to explore the effects of different drying methods on the volatile components of flower tea of Camellia sinensis. The volatile components of tea flowers processed by four different drying methods, including stir-frying drying (SFD), hot air drying (HAD), microwave drying (MD) and vacuum drying (VD), were comprehensively analyzed by sensory evaluation, electronic nose and HS-SPME-GC-MS. The results showed that there were significant differences in the effects of different drying methods on volatile components of tea flowers, with sweet aroma of SFD, grassy aroma of HAD, honey aroma of MD and fruity aroma of VD. The results of electronic nose showed that the aroma of HAD and SFD flower tea was similar. A total of 113 aroma components were detected by HS-SPME-GC-MS, including 25 aldehydes, 44 alcohols, 22 ketones, 9 esters, 10 hydrocarbons and 3 other compounds. The total volatile components were HAD > MD > VD > SFD. Through PLS-DA and difference significance analysis, 22 important significant markers for the difference of volatile components of flower tea dried by different drying methods were screened out, and 15 key substances were screened out in combination with odor activity value (OAV > 1), including α-phenylethanol, hexanal, acetophenone, linalool, heptanal, 2-methylbutanal, 1-penten-3-one, (E)-2-octenal,furfural, (Z)-linalool oxide (furanoid), 1-nonanol, nonanal, (Z)-linalool oxide (pyranoid ), geraniol, α-ionone. In addition, the flower tea processed by the four drying methods contained α-ionone, 2-methylbutanal, (E,E)-2,4-decadienal and 1-octen-3-ol, and the OAV was greater than 10. These aroma substances are the key volatile components of tea flower tea. The volatile components of flower tea processed by different drying methods were similar in species, but significantly different in content. In terms of the total amount of volatile components, HAD is beneficial to the production and accumulation of volatile components in flower tea of Camellia sinensis.