Abstract: Yunyan 87 was used as the material. The experiment adopted a randomized block design, with tobacco-specific base fertilizer（CK） as the control. Three treatments were set up including tobacco-specific base fertilizer + rapeseed cake organic fertilizer + Xin Ye organic fertilizer（T₁）, tobacco-specific base fertilizer + rapeseed cake organic fertilizer + soybean cake organic fertilizer（T₂）, and tobacco-specific base fertilizer + rapeseed cake organic fertilizer + sesame cake fertilizer（T₃）. The effects of different organic fertilizer combinations on soil physical and chemical properties and microbial community in flue-cured tobacco fields in Xiajiang were studied. The results showed that the addition of different types of organic fertilizers could increase soil available nutrients, microbial quantity, soil enzyme activity and bacterial community diversity, facilitate the enrichment of beneficial bacteria such as Sphingomonas, and inhibit the reproduction of pathogenic bacteria, thereby improving soil microbial structure. The T₃ treatment with the addition of sesame cake fertilizer was the best.