高级检索

高产优质香稻万香丝苗穗部性状QTLs鉴定分析

Identification and Analysis of QTLs for Panicle Traits of High-yield and High-quality Fragrant Rice Variety Wangxiangsimiao

  • 摘要: 以高产优质香稻万香丝苗和北方典型高产粳稻沈农9816为亲本杂交衍生的F2、F2:3群体为试验材料,鉴定穗长、穗粒数、结实率和千粒重等穗部性状并进行QTL定位。结果表明:(1)双亲穗部性状存在显著差异,F2、F2:3群体各家系穗部表型性状存在明显差异且呈现双向超亲分离,表现为近似正态分布。(2)检测到32个QTLs,分布于1~3、5和7~12号染色体上,表型贡献率在3.30%~36.91%之间,贡献率超过10.00%的主效QTLs有12个,有10个QTLs在连续2年试验中被重复定位到。(3)穗部性状QTL在染色体上呈现簇状分布,4个主效QTL簇分别为qGNP2qGNP5qGNP8qGNP9,其中qGNP5qGNP9所在染色体区域存在多个稳定表达的主效QTLs。研究鉴定发掘了多个稳定表达的主效QTL簇,这可为相关QTL精细定位和功能挖掘奠定基础,也为分子标记辅助选择培育高产水稻提供新的基因元件。

     

    Abstract: The F2 and F2:3 populations derived from high-yield and high-quality fragrant rice variety Wanxiangsimiao and northern high-yield japonica rice variety Shennong 9816 as experimental materials. We evaluated panicle traits including panicle length, number of grains per panicle, seed filling rate, thousand seeds weight, etc., and then conducted QTL mapping. The results showed that:(1) There were significant differences in the panicle traits of both parents. In the F2 and F2:3 populations, the panicle phenotypic traits of family lines showed obvious differences and exhibited bidirectional parental segregation, presenting an approximately normal distribution.(2) A total of 32 QTLs were identified on the chromosomes 1~3, 5, and 7~12, contributing 3.30% to 36.91% of the phenotypic variance. There were 12 main QTLs with a contribution rate exceeding 10.00%, and 10 QTLs were repeatedly identified in different environments in 2 years.(3) QTLs for panicle traits exhibited clustered distribution. Four QTL clusters qGNP2, qGNP5, qGNP8 and qGNP9, and then qGNP5 and qGNP9 contained multiple stable major QTLs. In this research, several main QTL clusters were identified, which can lay a foundation for the fine mapping and functional research of these QTL and provide new gene elements for molecular marker-assisted selection of high-yield rice varieties.