Abstract: Using‘Piaohe Erhao’ (a major sun-cured red tobacco variety) from Jiaohe City, Jilin Province as test material, a comparative experiment was conducted to evaluate the mitigating effect of herbicide antidotes, plant growth regulators, watersoluble fertilizers and microbial inoculants on herbicide damage, and their effect on the growth of tobacco plants, soil nutrients and enzyme activities were explored. The results showed that Diethyl aminoethyl hexanoate and Nai’an exhibited significant mitigation effects. The Nai’an treatment (T₄) resulted in the lowest herbicide injury index (3.70) and the highest alleviation effect (57.09%). This treatment also showed optimal performance in plant height, leaf area and root development. Furthermore, it increased yield by 65.27% and output value by 70.09% compared to the control. Bacillus subtilis and Trichoderma harzianum inoculants (T₅ and T₆) significantly increased soil available nutrient content and soil enzyme activities. However, their efficacy in mitigating herbicide injury was limited. In conclusion, Diethyl aminoethyl hexanoate and Nai’an are recommended as primary mitigating agents for herbicide phytotoxicity in tobacco fields. Application via root dipping or foliar spraying effectively can promote plant growth and enhance yield. Bacillus subtilis and Trichoderma harzianum are more suitable for improving soil fertility and are recommended for application via root dipping combined with root irrigation.