Abstract: The ameliorative effects of tea polyphenols (TP) on lipopolysaccharide (LPS)-induced intestinal inflammation in broilers were studied. A total of 240 one-day-old white feather broilers were randomly allocated into four experimental groups: control group (basal diet), LPS group (basal diet+LPS), TP group (basal diet+350 mg/kg TP), TP+LPS group (basal diet+350 mg/kg TP+LPS )。From day 21 to day 26 post-hatching, broilers in the LPS and TP+LPS groups received daily intraperitoneal injections of LPS. The results showed that LPS challenge significantly elevated serum levels of pro-inflammatory cytokines (IL-1β, IL-6) and tumor necrosis factor-α (TNF-α), which were effectively mitigated by TP supplementation. Notably, TP supplementation exhibited significant interactive effects with LPS challenge on modulating serum monocyte chemoattractant protein-1 (MCP-1) levels. LPS exposure significantly reduced the relative abundance of Firmicutes and Bacteroidetes phyla in the cecal microbiota. TP supplementation not only restored the Firmicutes/Bacteroidetes ratio but also significantly increased the abundance of beneficial genera including Alistipes and Bacteroides. Furthermore, LPS stimulation and dietary TP have a significant interactive effect on increasing cecal Alistipes and decreasing cecal Clostridia_UCG-014 in broilers. These findings suggest that dietary TP, as a natural phytogenic feed additive, can effectively attenuate intestinal inflammatory responses, restore intestinal microbial homeostasis, and promote the healthy development of the broiler breeding industry.