Abstract: Amorphophallus konjac is one of the important cash crops in the southwest mountinous area of China, but the resistance of most varieties and local species has declined seriously due to long-term asexual reproduction. Conventional clonal selection and hybrid breeding progress slowly in developing new varieties due to germplasm resource scarcity, prolonged flowering cycles, asynchronous flowering times between species and crossbreeding incompatibility issues, which makes it difficult to meet market demand for high-quality and disease-resistant cultivars. This paper reviewed key aspects of Amorphophallus konjac biotechnology, including explant selection, culture medium optimization, in vitro morphogenesis pathways, genetic transformation, mutagenesis and somaclonal variation screening, aiming to provide references for biological breeding research of Amorphophallus konjac.