Abstract: Five multiple planting years (CK, CR₃, CR₅, CR₇ and CR₁₀) were set up in the typical promotion contiguous demonstration area of tobacco and radish in Shiping County to explore the change characteristics of soil nutrients after different multiple planting years and their effects on tobacco yield and quality. The results showed that soil pH showed a significant downward trend, with tobacco-radish continuous planting. The tobacco grew well with better biomass, yield and chemical composition coordination in multiple planting 3 years, 5 years compared with cropping 1 year. Redundancy analysis (RDA) showed that soil pH value and available phosphorus content were the main factors causing the decline in tobacco yield. The soil chlorine content has a negative correlation with the total sugar content, reducing sugar content and potassium-chlorine ratio of tobacco, becoming the main factors affecting the tobacco quality. In conclusion, it could ensure better tobacco yield and quality within 7-years tobacco-radish planting. It was recommended that the multiple planting period of tobacco and radish should be less than 7 years.