Abstract: The tomato seedlings were used as test materials, and the effects of different concentrations (0, 50, 100, 150, 200 and 250 mg/L) of exogenous glucose on the growth and physiological indicators of tomato seedlings under drought stress were studied. The results revealed that compared to the control group (no glucose under drought stress), glucose treatment generally enhanced plant height, stem diameter and chlorophyll content. Notably, the 100 mg/L glucose treatment resulted in the most significant increases in these parameters, with the increases of 31.9%, 44.2% and 52.9%, respectively. Conversely, glucose application led to a reduction in root-shoot ratio, relative conductivity and malondialdehyde content, with the most pronounced decreases observed at 100 mg/L glucose, reaching 61.3%, 11.4%, and 58.6%, respectively. In conclusion, the application of 100 mg/L glucose can significantly alleviate the inhibitory effect of drought stress on the growth and development of tomato seedlings.