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5个甘薯品种对干旱胁迫的响应及耐旱品种筛选

Response of 5 Sweet Potato Varieties to Drought Stress and Selection of Drought-tolerant Varieties

    摘要
  • 摘要: 以普薯32、莆薯12、福菜薯18、福薯604和广薯87为试材，利用20% PEG-6000模拟连续干旱对不同甘薯品种幼苗的LCC、LAI、SLA、LWC、MDA含量，SOD、POD、CAT活性，植物激素含量和耐旱基因荧光定量表达水平进行了分析评价。结果表明：PEG-6000干旱胁迫7 d甘薯幼苗叶片的LCC、LAI和LWC下降，SLA、抗氧化酶活性和MDA含量增加，福薯604的LCC、LAI和LWC下降幅度最低，均为5%，SLA增幅最大，为169%，SOD、POD和CAT活性增幅显著，MDA含量增幅最小。在植物激素含量变化中，福薯604的IAA、IBA含量分别显著增加了15%和23%，ABA含量显著增加了69%，JA含量显著降低了84%，SA含量显著增加了36%，GA4含量降幅最小，为4%，GA7含量显著增加272%。干旱胁迫7 d，除广薯87外，其他甘薯品种IbWRKY2均下调表达，IbGATA24均上调表达，其中IbWRKY2在福菜薯18中降幅最小，IbGATA24在福薯604中增幅最大；除福薯604外，IbSDT在其他甘薯品种中均下调表达。综上，不同甘薯品种对干旱胁迫响应不同，福薯604耐旱基因表达水平和抗氧化酶活性增幅高，MDA增幅小，具有较强的耐旱性。

     

    Abstract: The sweet potato varieties including Pushu 32, Pushu 12, Fucaishu 18, Fushu 604, and Guangshu 87 were used as the test materials, and the chlorophyll content (LCC), leaf area index (LAI), leaf area ratio (SLA), leaf water content (LWC), malondialdehyde (MDA) content, superoxide dismutase (SOD) activity, peroxidase (POD) activity, catalase (CAT) activity, plant hormone content, and drought resistance gene fluorescence quantification levels were analyzed and evaluated under continuous drought stress using 20% PEG-6000. The results showed that under PEG-6000 drought stress for 7 days, the LCC, LAI, and LWC of sweet potato seedlings decreased, while the SLA, antioxidant enzyme activity, and MDA content increased. Fushu 604 had the lowest decrease in LCC, LAI and LWC, all by 5%, with the largest increase in SLA at 169%. The activities of SOD, POD and CAT increased significantly, while the MDA content decreased the least. In terms of changes in plant hormone content, the indole-3-acetic acid (IAA) and indole-butanoic acid (IBA) contents of Fushu 604 increased significantly by 15% and 23%, respectively, while the abscisic acid (ABA) content increased significantly by 69%, the jasmonic acid (JA) content decreased significantly by 84%, and the salicylic acid (SA) content increased significantly by 36%. The gibberellin A4 (GA4) content decreased the least, by 4%, and the gibberellin A7 (GA7) content increased significantly by 272%. Under drought stress for 7 days, the expression of IbWRKY2 was downregulated in all sweet potato varieties except Guangshu 87, while IbGATA24 was upregulated in all varieties. Among them, IbWRKY2 showed the smallest decrease in Fucaishu 18, and IbGATA24 had the largest increase in Fushu 604. IbSDT was downregulated in all sweet potato varieties except Fushu 604. In summary, different sweet potato varieties respond differently to drought stress. Fushu 604 has a high level of drought resistance gene expression and a high increase in antioxidant enzyme activity, a small increase in MDA, indicating strong drought tolerance.

     

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