Abstract: Based on field survey data from key rural vitalization counties in Guangxi, Logit model was used to analyze the factors influencing the agricultural variety demand, production technology demand and operation and management demand of small and micro agricultural enterprises in poverty alleviation areas. The results showed that the order of agricultural science and technology demand of small and micro agricultural enterprises was agricultural production technology, management information and agricultural variety information. The education level of the operating subject, industry type, enterprise scale, whether to carry out brand registration, whether to carry out product certification, and whether to obtain technical support were the main factors affecting the technical demand of small and micro agricultural enterprises. The improvement of education level of the management subject had a great promoting effect on the popularization of production technology and management technology. The processing industry had a stronger demand for agricultural technology than the breeding industry and the planting industry, and the breeding industry had a stronger demand for agricultural technology than the planting industry. Small and micro agricultural enterprises with larger scale, non-technical support and more attention to brand building had stronger demand for agricultural technology. According to the analysis, some countermeasures including improvement of the quality of operators of small and micro agricultural enterprises, carrying out product certification to cultivate brand, expanding the coverage of science and technology services in poverty alleviation areas, improvement of agricultural science and technology service system, construction of a service model for the whole industrial chain, innovation of the way of providing science and technology service, were proposed, in order to promote the precise matching of science and technology services with demand and provide strongscientific and technological support for the sustainable development of agricultural in poverty alleviation areas.