Abstract: In order to investigate the change laws of endogenous hormones in mango during early flowering period in winter, the flower buds (flower buds expanded and scales staggered), buds (calyx blooming and petals exposed) and blooming flowers (50% of the flowers of the whole tree open) of 5 different mango varieties at early flowering stage in winter were used as materials. The contents of auxin (IAA), abscisic acid (ABA), gibberellin (GA₃), zeatin (ZT) and jasmonic acid (JA) were determined by liquid chromatography-tandem mass spectrometry. The results showed that the changing trends of IAA, ABA and GA₃ were the same, and they all increased first and then decreased, reaching the highest at squaring stage. The contents of ABA and JA remained at a high level during the whole period, while the contents of IAA, GA3 and ZT were relatively low. There are differences in the ratio of each hormone, and the ratio of ABA/IAA, ZT/GA₃ and ZT/IAA showed a decreasing trend. The ratio of ABA/ZT and ABA/ GA₃ showed a trend of increasing at first and decreasing. In conclusion, ABA and JA can promote the early flowering of mango in winter, and the accumulation of IAA and GA3 is beneficial to the formation of buds. The high ratio of ABA/IAA, ZT/GA3 and ZT/ IAA is beneficial to the flower bud differentiation of mango, while the lower ratio is beneficial to flowering. The higher ratio of ABA/ZT and ABA/GA3 promotes the bud formation of mango. The results of this study can provide reference for the management and regulation of mango during flowering period in winter.