Abstract: This study investigated the effects of eight different root induction materials (hormones, functional fertilizers and microbial agents) on the root biomass and its distribution characteristics of Ficus altissima. The results showed that the different root induction materials had a significant impact on the aboveground growth parameters (plant height, crown width, root collar diameter and SPAD value) as well as the root biomass and morphological characteristics (root length, root surface area, root volume and root tip number). The T₂ treatment group (naphthalene acetic acid, indole-3-butyric acid, and trace elements) exhibited the best performance in promoting both aboveground biomass and root growth, with plant height increasing by 28.36 cm (a growth rate of 23.63%) and significant improvements in crown width and root morphology. The T₄ treatment group (mineral humic acid potassium, chitosan and microbial agents) showed outstanding results in increasing root dry weight. The T₃ treatment group (amino acids and calcium) significantly increased the total root length by 4192.77 cm, while the T₈ treatment group (microbial agents) significantly enhanced the root-shoot ratio. In conclusion, the hormone-based and humic acid-based materials hold promising application potential in the cultivation of Ficus altissima.