Abstract: Forty-one ratooning rice varieties with potential productivity were screened out, and the adaptability of growth period, yield and final regeneration rate was investigated. The index assignment was carried out by arithmetic average method to comprehensively evaluate the production potential of the test varieties as ratooning rice. The results showed that the growth period of all varieties was suitable for the requirements of ratooning rice planting in the Middle and lower reaches of the Yangtze River. Taoyou 77 and Taoyouxiangzhan matured in early October, which provided better utilization conditions for the three-cropping system. Twelve varieties such as Zhuliangyouhuizhan, Huazheyou 1, Yueliangyouyuzhan, Keliangyou 1019 and Weiliangyou 8612 were selected as the ratooning rice varieties for further demonstration in the Middle and lower reaches of the Yangtze River based on assignment data. The final regeneration rate was significantly and extremely significantly positively correlated with the yield of the regeneration season, total yield, total yield assignment and total assignment, which show that the quantificational evaluation method has strong feasibility and reference value.