Abstract: The samples were extracted with 80% acetonitrile solution, purified with PRiME HLB solid phase extraction column, concentrated with nitrogen, and finally dissolved with 10% methanol. A gradient elution was performed using 0.01% formic acid water-methanol as a mobile phase for gradient elution. The multi-reaction monitoring (MRM) mode of UPLC-MS/MS was used for the determination, and quantification was performed by internal standard method. The results showed that the concentration of 30 compounds showed good linearity in the range of 0.5～20.0 ng/mL, with the coefficient correlation (R²) between 0.993997 to 0.999872. At the addition levels of 1.0, 5.0 and 10.0 μg/kg, the recovery ratio of 30 compounds was 85.9%～115.8% in pork. The detection limit of 30 compounds was 0.02～1.00 μg/kg, and quantification limit was 0.06～3.00 μg/kg. The test indicated that the method was simple, sensitive, accurate, stable in recovery, good accuracy and repeatability, and suitable for the detection and analysis of large quantities of pork samples.