Abstract: In order to explore the differences and usability of routine chemical components in C3F tobacco leaves of five county-level production areas (Anqiu, Zhucheng, Linqu, Gaomi and Changle) and six varieties (NC55, YY301, ZC208, ZY100, ZY101 and YY87) in Weifang City, statistical methods such as multiple comparisons, cluster analysis, and fuzzy mathematics were used to analyzed six routine chemical components, including total sugar, reducing sugar, total nitrogen, nicotine, potassium, and chlorine. The results showed that: (1) The average contents of total sugar and reducing sugar in flue-cured tobacco in Weifang were in the optimal range, while the contents of total nitrogen, nicotine, and potassium were slightly lower, and the content of chlorine was higher. The coefficient of variation for the content of each chemical component ranged from 9.36% to 42.31%, with significant fluctuations. (2) The overall chemical components of tobacco leaves varied greatly among different regions in Weifang City, and had obvious regional distribution characteristics. The overall difference in the chemical components of tobacco leaves among different varieties was relatively small, and there were no obvious distribution characteristics of the varieties. This indicated that the influence of geographical ecological environment on the chemical components of tobacco leaves was greater than the differences in varieties. (3) There were certain differences in the chemical components usability index (CCUI) in tobacco leaves among different regions and varieties, with Gaomi region and YY87 variety performing better. The overall CCUI of tobacco in Weifang City was at a medium or above level. Based on the research results, it is recommended to adopt targeted agronomic regulation measures for some regions and varieties of tobacco in Weifang City to improve their tobacco quality and usability. At the same time, this study can provide scientific basis for the innovation of tobacco breeding and cultivation technology in Weifang City and similar ecological areas, and help to promote the efficient utilization of tobacco resources and the differentiated development of cigarette products.