Abstract: Yunyan 99 was used as the material in this experiment. Integrated the climate environment and cultivation system of Xixiang County, three treatments were set up: traditional equal row spacing conventional density cultivation mode (CK), traditional equal row spacing high-density cultivation mode (T₁) and wide narrow row high-density cultivation mode (T₂). The differences in agronomic traits, dry matter accumulation, appearance quality, chemical composition, yield quality, and economic benefits of flue-cured tobacco were studied under different treatments. The results showed that the overall agronomic traits and dry fresh weight of the three treatments were CK＞T₂＞T₁. The T₂ treatment could alleviate the effect of high density on the thin and weak growth of tobacco plants to a certain extent. High density treatments T₁ and T₂ reduced the fresh weight and dry matter accumulation of individual plants, and the dry weight decreased. The reduction effect of T₁ was significant. The appearance quality of the three treatments was greatly affected by the leaf position, with the middle leaf having the best appearance quality and the lower leaf having poorer appearance quality, with the upper leaf having better appearance quality. The changes in chemical composition of upper leaves and middle leaves were similar, with T₁ and T₂ treatments exhibiting high sugar and low alkali content, and the coordination of chemical composition was within an appropriate range. The yields of T₁ and T₂ were both higher than CK, increasing by 8.89% and 14.81% respectively. The output values of T₁ and T₂ increased by 3.50% and 16.84% respectively compared to CK. Comprehensive analysis shows that T₂ treatment has the best comprehensive benefits and is suitable for promotion and application in the Hanzhong tobacco-growing areas.